Biochemical characteristics and functional performance of cold-stored platelets: an in-vitro comparative study

Background: Platelet refrigeration could eliminate bacterial contamination and improve the hemostatic function even better than already-used room-temperature storage. This study aimed to assess effect of cold storage, with without agitation, on apheresis platelets' hemostatic, metabolic, functional activity. Materials methods: The included 10 healthy volunteer donors collect Apheresis PLT. They were submitted careful clinical examination standard laboratory workup. Collected samples processed in accordance American Association Blood Banks (AABB) guidelines. Every aliquot collected from each was stored for up 5 days at one following storage conditions: 1. In an FDA-approved-PLT incubator agitation room temperature (RT + AG as a group; GI), 2. FDA-approved-refrigerator 4 oC (4 GII), 3. FDA-approved- refrigerator – GIII). PLT workup done; count mean platelet volume (MPV), metabolic variables, aggregation studies, receptors expression, pro-inflammatory mediator’s release.Results:All had significant decline compared baseline data. No changes MPV observed all groups day 3 5, meaning that single size remained unchanged. addition, GI showed mark increase activity when PLTs contrast GII, GIII, which more metabolically stable less active.Comparison between studied regarding revealed significantly higher response ADP collagen GII GIII 3rd 5th days. Moreover, it shown CD62p expression despite being active stable. While found TXB2 levels higher, nearly 3-fold, GIII.Conclusions: platelets (AP) provides clear advantage over conditions biochemical balance performance, markedly AP's economic value different scenarios. Keywords: aggregation, P-selectin, Thromboxane B2.